Fluorescence Microscopes

NovaFLIM NEW

Fluorescence Lifetime Imaging Analysis Software

  • Imaging analysis with Luminosa, MicroTime 200, and LSM Upgrade Kits
  • Fast analysis of FLIM, FLIM-FRET, and anisotropy data, based on GPU-accelerated algorithms
  • Efficient batch analysis of z-stacks, time-lapse series and stitched images
  • Advanced, flexible ROI handling
  • Reproducible ROI selection incorporating histograms of fitted parameters
Image NovaFLIM Fluorescence Lifetime Imaging Analysis Software

Advanced analysis of images at your fingertips with the click of a button

Quality and precision you can trust

  • Multi-faceted view of your FLIM data by combining multi-exponential fitting, phasor plots, and pattern matching
  • Quantitative, robust, and reproducible ROI selection via 1D and 2D histograms or from phasor plot

ROI selection via 2D histogram of average lifetime vs. intensity for lifetime-based multiplexing

Save time and simply focus on your samples

  • Seamlessly integrated with Luminosa
  • High speed thanks to GPU-based algorithms
  • Minimal user interaction required
  • Efficient analysis of series measurements, including batch analysis and parameter plots  of multiple-ROIs

Parameter plot: relative amplitude of lifetime component 1 over frames of an image series

Advanced flexibility

  • Simultaneous batch analysis for FLIM, FLIM-FRET, anisotropy for user defined channels
  • Many data export options for graphs and images (Bitmap, PNG, OME TIFF, Scalable Vector Graphics)

FLIM image exported as SVG, to be modified in external graphics software

Highlights of NovaFLIM

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Licence type Software licence, available for either 1, 2, or 4 years
Data formats Files acquired with Luminosa
Files acquired with SymPhoTime 64 (version 2.5 from March 2020 or higher) using a MicroTime 200, MicroTime 100, or LSM Upgrade Kit
PC system requirements
  Minimum Recommended
Operating system Win10 64-bit Win10 / Win11 64-bit
CPU Intel Core i3-8100 or AMD Ryzen 3 1300X  Intel Core i7-13700 or AMD Ryzen 7 5700X
RAM  8 GB  32 GB
GPU AMD Radeon RX550/550 Series or Nvidia GeForce GT 1030 AMD Radeon RX 6700 XT or Nvidia GeForce RTX 3060
Screen resolution QHD (2560 × 1440) 4K (3840 × 2160)

NovaConvert - included software to convert *.spqr files and *.ptu files acquired with SymPhoTime 64 (version 2.3 from July 2017 or higher) to be analyzed in NovaFLIM.

NovaConvert screenshot: defining detection channels for converting *.ptu file acquired with SPT64 to *.spqr file for analysis with NovaFLIM 


All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

  Luminosa NovaFLIM
InstaFLIM
  • Fast GPU accelerated analysis with minimal user interaction
  • 4 different FLIM contrast options: Fast lifetime, multi-exponential decay fit, phasor plot, and pattern matching
  • Suggestion for FLIM species separation
  • Suggested parameters can be fine-tuned
  
Phasor plot for marker multiplexing  
Multi-frame analysis  
Batch analysis of series measurements (time-lapse,z-stacks, tiling, …)  
Advanced ROI handling, multi-ROIs, import of external ROIs  
Parameter plots of fitted parameters over multiple ROIs  
Representation of the image information as 
user defined 1D and 2D histograms of fitted parameters		  
ROI selection via parameter histograms  
User configured analyses  allowing for
FLIM, FLIM-FRET, steady-state FRET and anisotropy imaging		  
Extended export options  of graphs and images   
Image Scanning Microscopy-FLIM 
(Resolution enhancement via a pixel reassigned image 
available in combination with PDA-23 Add-on)		  
Re-assignement vectors calculation from image  


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