Book on Advanced Photon Counting
Advanced Photon Counting
Applications, Methods, Instrumentation
Editors: Peter Kapusta, Michael Wahl, Rainer Erdmann
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Table of contents
- Modern TCSPC Electronics: Principles and Acquisition Modes
Michael Wahl - Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm
Andreas Bülter - Single-Photon Detectors for Infrared Wavelengths in the Range 1–1.7 µm.
Gerald S. Buller and Robert J. Collins - Modern Pulsed Diode Laser Sources for Time-Correlated Photon Counting
Thomas Schönau, Sina Riecke, Andreas Bülter, and Kristian Lauritsen - Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS
Thomas Dertinger and Steffen Rüttinger - Lifetime-Weighted FCS and 2D FLCS: Advanced Application of Time-Tagged TCSPC
Kunihiko Ishii, Takuhiro Otosu, and Tahei Tahara - MFD-PIE and PIE-FI: Ways to Extract More Information with TCSPC
Anders Barth, Lena Voith von Voithenberg, and Don C. Lamb - Photon Antibunching in Single Molecule Fluorescence Spectroscopy
Kristin S. Grußmayer and Dirk-Peter Herten - FLIM Strategies for Intracellular Sensing
Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, and Angel Orte - Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue
Rafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, and Zygmunt Gryczynski - Pattern-Based Linear Unmixing for Efficient and Reliable Analysis of Multicomponent TCSPC Data
Ingo Gregor and Matthias Patting - Metal-Induced Energy Transfer
Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, and Alexey I. Chizhik - The Importance of Photon Arrival Times in STED Microscopy
Giuseppe Vicidomini, Ivan Coto Hernandez, Alberto Diaspro, Silvia Galiani, and Christian Eggeling - Single-Color Centers in Diamond as Single-Photon Sources and Quantum Sensors
Boris Naydenov and Fedor Jelezko - Photon Counting and Timing in Quantum Optics Experiments
Andreas Ahlrichs, Benjamin Sprenger, and Oliver Benson - Photon Counting in Diffuse Optical Imaging
Dirk Grosenick
More details including abstracts and previews are available on the dedicated website at Springer.