Time-Resolved Spectroscopy for Bioanalytical Applications

Time-Resolved Spectroscopy for Bioanalytical Applications: Advanced Time-Domain Fluorescence Spectroscopy and Microscopy (FLIM, FRET, FCS & SMD)

Thursday, August 30, 2007

Joint workshop between PicoQuant GmbH and the Center for Commercialization of Fluorescence Technologies at the University of North Texas Health Science Center

General
Speakers
Schedule
Archive

Summary

The workshop was dedicated to interested students and scientists from Life Sciences having a certain background in fluorescence and confocal microscopy and looking to extend their knowledge to the benefits of time-resolved methods. In the afternoon we provided the opportunity to see state-of-the-art instruments working and gave a detailed explanation of key features by highly recognized instructors.

Product demos

Product demos that where offered included:

MicroTime 200: time-resolved confocal microscope with single molecule sensitivity
FluoTime 200: modular fluorescence lifetime spectrometer
SPCE-Setup: Surface Plasmons Assisted Microscopy (SPAM) combines time-resolved microscopy with nanotechnologies, utilizing surface plasmon resonance (SPR) excitation and surface plasmons-coupled emission (SPCE).

Lecturers / Instructors

Julian Borejdo, Professor in the Department of Molecular Biology and Immunology in UNTHSC.
Ignacy Gryczynski, Professor and Director of Microscopy Core Facility in the Department of Cell Biology and Genetics in UNTHSC.
Zygmunt (Karol) Gryczynski, Professor in the Department of Molecular Biology and Immunology in UNTHSC.
Samantha Fore, Post-doc at the Center for Biophotonics, UC Davis, Sacramento.
Thomas Dertinger, Post-doc at the group of Shimon Weiss, UCLA.
Peter Kapusta, Senior Scientist at PicoQuant.
Uwe Ortmann, Head of Sales and Marketing at PicoQuant.

Time schedule

Time	Speaker	Title of lecture / talk
8:30	Karol Gryczynski / Uwe Ortmann	Welcome & short introduction of UNTHSC / PQ
8:45	Peter Kapusta	The FluoTime Series: a versatile family of TCSPC based fluorescence spectrometer
9:30	Karol & Ignacy Gryczynski	FluoTime: time-resolved measurements, fluorescence lifetime standards, limits of resolution
10:00	Coffee break
10:20	Uwe Ortmann	The MicroTime Series: a versatile family of TCSPC based confocal fluorescence microscopes with single molecule sensitivity for FCS, FRET & FLIM
10:50	Samantha Fore	Time-resolved fluorescence spectroscopy applications in biology using the MicroTime 200 - from single molecules to living organisms
11:30	Thomas Dertinger	Two focus FCS: a new tool for ultraprecise measurements
12:10	Karol Gryczynski / Julian Borejdo	Single molecule conformational dynamics using Surface Plasmons Assisted Microscopy (SPAM)
12:50	Lunch (at the Museum - for all participants)
14:30	Product demo 1	FluoTime 200, MicroTime 200 and SPCE setup
15:20	Product demo 2	FluoTime 200, MicroTime 200 and SPCE setup
16:10	Coffee break
16:40	Product demo 3	FluoTime 200, MicroTime 200 and SPCE setup
18:00	Beer & Pizza reception (Room 434 - for participants of product demonstrations only)